Journal: Scientific Reports

Article Title: Development and characterization of an inducible Tensin1 deficient transgenic murine model

doi: 10.1038/s41598-026-41319-3

Figure Lengend Snippet: Generation of Tns1 lox, lox mouse for conditional TNS1 knockout. ( A ) Schematic of Tns1 gene locus on Chromosome 1. The targeted exon is expressed in five commonly translated variants. ( B ) Schematic of targeted exon with approximate location of LoxP sites, surrounding exons, and polymerase chain reaction (PCR) primers. C-H. Rosa Cre/+ ; Tns1 lox/lox (Tns1-/-) or TNS1-expressing wild type controls (Tns1+/+) from either B6.Tns1 lox, lox or Rosa Cre/+ ; Tns1 +/+ were treated with daily tamoxifen. ( C ) Gel electrophoresis of tail DNA PCR product confirms excised exon. Real time quantitative PCR of Tns1 ( D ), Tns2 ( E ), and Tns3 ( F ) mRNA expression in lung tissue. Western blot ( G ) and associated densitometry ( H ) in lung tissue ( n = 3 biological repeats). Unpaired Student’s t -test was utilized for all statistical assessments (* p < 0.05). Data is presented as mean ± SD.

Article Snippet: Bulk RNA sequencing analysis was then performed using the Illumina NovaSeq6000 S4 System (2 × 150 bp, ~ 66 M reads/sample) with the Illumina TruSeq Stranded mRNA (polyA enrichment) Library.

Techniques: Knock-Out, Polymerase Chain Reaction, Expressing, Nucleic Acid Electrophoresis, Real-time Polymerase Chain Reaction, Western Blot